Molecular Biology

The application of molecular markers to assess seed orchard mating dynamics and orchard management procedures. This research includes evaluating genetic diversity of orchard crops using molecular markers as well as confirming the underlying assumptions of our seedlot rating protocol. PCR-amplified chloroplast DNA markers are the markers of choice as they are inherited in conifers through the male gamete (pollen). These chloroplast markers are now available for most commercial British Columbia conifers. Other areas of applications of these markers include the determination of mating dynamics in orchard (rate of outcrossing, amount of nearest neighbour matings, selfing levels) and the determination of pollen contamination and supplemental mass pollination success. For published papers describing the development and application of DNA markers to important B.C. conifers and seed orchard issues - click here.